human osteoblast hfob1 19 Search Results


human fetal osteoblast cells  (ATCC)
96
ATCC human fetal osteoblast cells
Human Fetal Osteoblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human osteoblast cells line hfob 1.19  (Lonza)
90
Lonza human osteoblast cells line hfob 1.19
( A ) ALP activity showed an increase of calcification of the extracellular matrix after inclusion of GelMA. ( B ) Calcium deposition demonstrated a further influence of GelMA to enhance the functions of <t>osteoblasts.</t> ( C ) MTS assay showing that osteoblastic cells were further influenced by hydrophilic properties after inclusion of PEG and GelMA. Data plotted in mean and SD (N=5). Values of P <0.01 were considered significant. Data were normalized by the cells, and the y-axis was multiplied by 10 4 . For the ALP and calcium deposition, the data were compared to control (cells) and between each time. For cellular proliferation assays, the data were compared to pure PCL. N=5. ** P <0.01, *** P <0.001, and **** P <0.0001 mean statistical differences. SEM of hFOBs cultivated on scaffolds after 7 days. ( D ) (i) PCL and (ii) magnified view. ( E ) (i) PCL-PEG and (ii) magnified view. ( F ) (i) PCL-PEG-GelMA without UV crosslinking and (ii) magnified view. ( G ) (i) PCL-PEG-GelMA after UV crosslinking and (ii) magnified view. The cells are spreading on all produced scaffolds presenting filopodium and cytoplasmic extension. Abbreviations: ALP, alkaline phosphatase; GelMA, gelatin methacryloyl; hFOB, human osteoblasts; MTS, (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium); NS, no significance; PCL, polycaprolactone; PEG, poly(ethylene glycol); SEM, scanning electron microscopy.
Human Osteoblast Cells Line Hfob 1.19, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
human osteoblast cells line hfob 1.19 - by Bioz Stars, 2026-06
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human osteoblasts hfob 1.19  (IOtech Inc)
90
IOtech Inc human osteoblasts hfob 1.19
( A ) ALP activity showed an increase of calcification of the extracellular matrix after inclusion of GelMA. ( B ) Calcium deposition demonstrated a further influence of GelMA to enhance the functions of <t>osteoblasts.</t> ( C ) MTS assay showing that osteoblastic cells were further influenced by hydrophilic properties after inclusion of PEG and GelMA. Data plotted in mean and SD (N=5). Values of P <0.01 were considered significant. Data were normalized by the cells, and the y-axis was multiplied by 10 4 . For the ALP and calcium deposition, the data were compared to control (cells) and between each time. For cellular proliferation assays, the data were compared to pure PCL. N=5. ** P <0.01, *** P <0.001, and **** P <0.0001 mean statistical differences. SEM of hFOBs cultivated on scaffolds after 7 days. ( D ) (i) PCL and (ii) magnified view. ( E ) (i) PCL-PEG and (ii) magnified view. ( F ) (i) PCL-PEG-GelMA without UV crosslinking and (ii) magnified view. ( G ) (i) PCL-PEG-GelMA after UV crosslinking and (ii) magnified view. The cells are spreading on all produced scaffolds presenting filopodium and cytoplasmic extension. Abbreviations: ALP, alkaline phosphatase; GelMA, gelatin methacryloyl; hFOB, human osteoblasts; MTS, (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium); NS, no significance; PCL, polycaprolactone; PEG, poly(ethylene glycol); SEM, scanning electron microscopy.
Human Osteoblasts Hfob 1.19, supplied by IOtech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
human osteoblasts hfob 1.19 - by Bioz Stars, 2026-06
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human normal osteoblast hfob 1.19  (ScienCell)
90
ScienCell human normal osteoblast hfob 1.19
( A ) ALP activity showed an increase of calcification of the extracellular matrix after inclusion of GelMA. ( B ) Calcium deposition demonstrated a further influence of GelMA to enhance the functions of <t>osteoblasts.</t> ( C ) MTS assay showing that osteoblastic cells were further influenced by hydrophilic properties after inclusion of PEG and GelMA. Data plotted in mean and SD (N=5). Values of P <0.01 were considered significant. Data were normalized by the cells, and the y-axis was multiplied by 10 4 . For the ALP and calcium deposition, the data were compared to control (cells) and between each time. For cellular proliferation assays, the data were compared to pure PCL. N=5. ** P <0.01, *** P <0.001, and **** P <0.0001 mean statistical differences. SEM of hFOBs cultivated on scaffolds after 7 days. ( D ) (i) PCL and (ii) magnified view. ( E ) (i) PCL-PEG and (ii) magnified view. ( F ) (i) PCL-PEG-GelMA without UV crosslinking and (ii) magnified view. ( G ) (i) PCL-PEG-GelMA after UV crosslinking and (ii) magnified view. The cells are spreading on all produced scaffolds presenting filopodium and cytoplasmic extension. Abbreviations: ALP, alkaline phosphatase; GelMA, gelatin methacryloyl; hFOB, human osteoblasts; MTS, (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium); NS, no significance; PCL, polycaprolactone; PEG, poly(ethylene glycol); SEM, scanning electron microscopy.
Human Normal Osteoblast Hfob 1.19, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mg63 osteosarcoma cells  (Procell Inc)
86
Procell Inc mg63 osteosarcoma cells
Cell viability (%) of <t>MG63</t> and Saos-2 cells treated with pexidartinib (via PLX3397) and sotuletinib.
Mg63 Osteosarcoma Cells, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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osteoblasts human source  (ATCC)
93
ATCC osteoblasts human source
Cell viability (%) of <t>MG63</t> and Saos-2 cells treated with pexidartinib (via PLX3397) and sotuletinib.
Osteoblasts Human Source, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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osteoblasts human source - by Bioz Stars, 2026-06
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human osteoblast hfob1 19  (Servicebio Inc)
86
Servicebio Inc human osteoblast hfob1 19
The role of miR-27a-3p in osteosarcoma cells. (A) Expression levels of miR-27a-3p in normal <t>osteoblasts</t> <t>hFOB1.19</t> and osteosarcoma cells Saos-2 and MG-63. (B,C) The influence of introducing miR-27a-3p mimic and inhibitor on the expression of miR-27a-3p in osteosarcoma cell lines MG-63 and SAOS-2. (D-F) CCK-8 assay, Transwell migration assay and FCM assay were used to detect the changes in cell proliferation, migration ability and apoptosis levels of osteosarcoma cells MG-63 and Saos-2 transfected with miR-27a-3p mimic and inhibitor. Stained with crystal violet; magnification: ×200. *, P<0.05; **, P<0.01. CCK-8, Cell Counting Kit-8; FCM, flow cytometry; NC, negative control.
Human Osteoblast Hfob1 19, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human fetal osteoblast cell line hfob1.19  (LGC Promochem)
90
LGC Promochem human fetal osteoblast cell line hfob1.19
The role of miR-27a-3p in osteosarcoma cells. (A) Expression levels of miR-27a-3p in normal <t>osteoblasts</t> <t>hFOB1.19</t> and osteosarcoma cells Saos-2 and MG-63. (B,C) The influence of introducing miR-27a-3p mimic and inhibitor on the expression of miR-27a-3p in osteosarcoma cell lines MG-63 and SAOS-2. (D-F) CCK-8 assay, Transwell migration assay and FCM assay were used to detect the changes in cell proliferation, migration ability and apoptosis levels of osteosarcoma cells MG-63 and Saos-2 transfected with miR-27a-3p mimic and inhibitor. Stained with crystal violet; magnification: ×200. *, P<0.05; **, P<0.01. CCK-8, Cell Counting Kit-8; FCM, flow cytometry; NC, negative control.
Human Fetal Osteoblast Cell Line Hfob1.19, supplied by LGC Promochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) ALP activity showed an increase of calcification of the extracellular matrix after inclusion of GelMA. ( B ) Calcium deposition demonstrated a further influence of GelMA to enhance the functions of osteoblasts. ( C ) MTS assay showing that osteoblastic cells were further influenced by hydrophilic properties after inclusion of PEG and GelMA. Data plotted in mean and SD (N=5). Values of P <0.01 were considered significant. Data were normalized by the cells, and the y-axis was multiplied by 10 4 . For the ALP and calcium deposition, the data were compared to control (cells) and between each time. For cellular proliferation assays, the data were compared to pure PCL. N=5. ** P <0.01, *** P <0.001, and **** P <0.0001 mean statistical differences. SEM of hFOBs cultivated on scaffolds after 7 days. ( D ) (i) PCL and (ii) magnified view. ( E ) (i) PCL-PEG and (ii) magnified view. ( F ) (i) PCL-PEG-GelMA without UV crosslinking and (ii) magnified view. ( G ) (i) PCL-PEG-GelMA after UV crosslinking and (ii) magnified view. The cells are spreading on all produced scaffolds presenting filopodium and cytoplasmic extension. Abbreviations: ALP, alkaline phosphatase; GelMA, gelatin methacryloyl; hFOB, human osteoblasts; MTS, (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium); NS, no significance; PCL, polycaprolactone; PEG, poly(ethylene glycol); SEM, scanning electron microscopy.

Journal: International Journal of Nanomedicine

Article Title: Electrospun nanofiber blend with improved mechanical and biological performance

doi: 10.2147/IJN.S175619

Figure Lengend Snippet: ( A ) ALP activity showed an increase of calcification of the extracellular matrix after inclusion of GelMA. ( B ) Calcium deposition demonstrated a further influence of GelMA to enhance the functions of osteoblasts. ( C ) MTS assay showing that osteoblastic cells were further influenced by hydrophilic properties after inclusion of PEG and GelMA. Data plotted in mean and SD (N=5). Values of P <0.01 were considered significant. Data were normalized by the cells, and the y-axis was multiplied by 10 4 . For the ALP and calcium deposition, the data were compared to control (cells) and between each time. For cellular proliferation assays, the data were compared to pure PCL. N=5. ** P <0.01, *** P <0.001, and **** P <0.0001 mean statistical differences. SEM of hFOBs cultivated on scaffolds after 7 days. ( D ) (i) PCL and (ii) magnified view. ( E ) (i) PCL-PEG and (ii) magnified view. ( F ) (i) PCL-PEG-GelMA without UV crosslinking and (ii) magnified view. ( G ) (i) PCL-PEG-GelMA after UV crosslinking and (ii) magnified view. The cells are spreading on all produced scaffolds presenting filopodium and cytoplasmic extension. Abbreviations: ALP, alkaline phosphatase; GelMA, gelatin methacryloyl; hFOB, human osteoblasts; MTS, (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium); NS, no significance; PCL, polycaprolactone; PEG, poly(ethylene glycol); SEM, scanning electron microscopy.

Article Snippet: Totally 5,000 cells/cm 2 of human osteoblast cells line (hFOB 1.19, bone-forming cells; Lonza, CRL-11372, second passage) were cultured using C27015 media (Osteoblast Basal Medium, Promocell GmbH), 1% penicillin/streptomycin (P/S; Hyclone), and an Osteoblast Growth Medium Supplement Mix under standard cell culture conditions (37°C, 5% CO 2 , and 95% air).

Techniques: Activity Assay, MTS Assay, Control, Produced, Electron Microscopy

Cell viability (%) of MG63 and Saos-2 cells treated with pexidartinib (via PLX3397) and sotuletinib.

Journal: Oncology Letters

Article Title: Computational discovery of novel colony-stimulating factor-1 receptor as a potential therapeutic biomarker in osteosarcoma and a novel inhibitor from herbal sources

doi: 10.3892/ol.2026.15618

Figure Lengend Snippet: Cell viability (%) of MG63 and Saos-2 cells treated with pexidartinib (via PLX3397) and sotuletinib.

Article Snippet: MG63 osteosarcoma cells were purchased from Procell Life Science Technology Co., Ltd. (cat. no. #CL-0157; http://www.procell.com.cn/p/mg-63-cl-0157-72575 ).

Techniques:

Sensitization of the MG63 and Saos-2 cell lines to a CSF1R inhibitor. (A) Dose-dependent cytotoxic effects of linarin on MG63 and Saos-2 osteosarcoma cell viability. (B) Dose-dependent cytotoxic effects of saikosaponin C on MG63 and Saos-2 osteosarcoma cell viability. (C) Dose-dependent cytotoxic effects of sarsasapogenin on MG63 and Saos-2 osteosarcoma cell viability. (D) Molecular docking of the binding between sarsasapogenin and the CSF1R protein. (E) Visual molecular docking of the binding acid residues between sarsasapogenin and CSF1R protein. (F) The expression levels of CSF1R as detected by western blot analysis in MG63 and Saos-2 cells after treatment with sarsasapogenin. *P<0.05. CSF1R, colony-stimulating factor-1 receptor.

Journal: Oncology Letters

Article Title: Computational discovery of novel colony-stimulating factor-1 receptor as a potential therapeutic biomarker in osteosarcoma and a novel inhibitor from herbal sources

doi: 10.3892/ol.2026.15618

Figure Lengend Snippet: Sensitization of the MG63 and Saos-2 cell lines to a CSF1R inhibitor. (A) Dose-dependent cytotoxic effects of linarin on MG63 and Saos-2 osteosarcoma cell viability. (B) Dose-dependent cytotoxic effects of saikosaponin C on MG63 and Saos-2 osteosarcoma cell viability. (C) Dose-dependent cytotoxic effects of sarsasapogenin on MG63 and Saos-2 osteosarcoma cell viability. (D) Molecular docking of the binding between sarsasapogenin and the CSF1R protein. (E) Visual molecular docking of the binding acid residues between sarsasapogenin and CSF1R protein. (F) The expression levels of CSF1R as detected by western blot analysis in MG63 and Saos-2 cells after treatment with sarsasapogenin. *P<0.05. CSF1R, colony-stimulating factor-1 receptor.

Article Snippet: MG63 osteosarcoma cells were purchased from Procell Life Science Technology Co., Ltd. (cat. no. #CL-0157; http://www.procell.com.cn/p/mg-63-cl-0157-72575 ).

Techniques: Binding Assay, Expressing, Western Blot

The role of miR-27a-3p in osteosarcoma cells. (A) Expression levels of miR-27a-3p in normal osteoblasts hFOB1.19 and osteosarcoma cells Saos-2 and MG-63. (B,C) The influence of introducing miR-27a-3p mimic and inhibitor on the expression of miR-27a-3p in osteosarcoma cell lines MG-63 and SAOS-2. (D-F) CCK-8 assay, Transwell migration assay and FCM assay were used to detect the changes in cell proliferation, migration ability and apoptosis levels of osteosarcoma cells MG-63 and Saos-2 transfected with miR-27a-3p mimic and inhibitor. Stained with crystal violet; magnification: ×200. *, P<0.05; **, P<0.01. CCK-8, Cell Counting Kit-8; FCM, flow cytometry; NC, negative control.

Journal: Translational Cancer Research

Article Title: Molecular mechanism of miR-27a-3p targeting FBXW7 regulating the malignant behavior of osteosarcoma cells

doi: 10.21037/tcr-2025-aw-2290

Figure Lengend Snippet: The role of miR-27a-3p in osteosarcoma cells. (A) Expression levels of miR-27a-3p in normal osteoblasts hFOB1.19 and osteosarcoma cells Saos-2 and MG-63. (B,C) The influence of introducing miR-27a-3p mimic and inhibitor on the expression of miR-27a-3p in osteosarcoma cell lines MG-63 and SAOS-2. (D-F) CCK-8 assay, Transwell migration assay and FCM assay were used to detect the changes in cell proliferation, migration ability and apoptosis levels of osteosarcoma cells MG-63 and Saos-2 transfected with miR-27a-3p mimic and inhibitor. Stained with crystal violet; magnification: ×200. *, P<0.05; **, P<0.01. CCK-8, Cell Counting Kit-8; FCM, flow cytometry; NC, negative control.

Article Snippet: Osteosarcoma cell lines MG-63 (RRID: CVCL_0426) and Saos-2 (RRID: CVCL_0548), as well as the normal human osteoblast hFOB1.19 (RRID: CVCL_3889), were sourced from Servicebio company (Wuhan, China).

Techniques: Expressing, CCK-8 Assay, Transwell Migration Assay, Migration, Transfection, Staining, Cell Counting, Flow Cytometry, Negative Control

The expression of FBXW7 in osteosarcoma cells. (A) The mRNA levels of FBXW7 in normal osteoblasts hFOB1.19 and osteosarcoma cells Saos-2, MG-63 were also analyzed. (B,C) To explore the impact of FBXW7 on the biological behavior of osteosarcoma cells Saos-2 and MG-63, the researchers performed transfections on these two osteosarcoma cell lines using pcDNA3.1-FLAG-FBXW7 and siRNA-FBXW7. (D,E) Effect of transfection of pcDNA3.1-FLAG-FBXW7 and siRNA-FBXW7 on expression of FBXW7 and β-catenin in osteosarcoma cells MG-63 (D) and Saos-2 (E). *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant. NC, negative control; OE, overexpression.

Journal: Translational Cancer Research

Article Title: Molecular mechanism of miR-27a-3p targeting FBXW7 regulating the malignant behavior of osteosarcoma cells

doi: 10.21037/tcr-2025-aw-2290

Figure Lengend Snippet: The expression of FBXW7 in osteosarcoma cells. (A) The mRNA levels of FBXW7 in normal osteoblasts hFOB1.19 and osteosarcoma cells Saos-2, MG-63 were also analyzed. (B,C) To explore the impact of FBXW7 on the biological behavior of osteosarcoma cells Saos-2 and MG-63, the researchers performed transfections on these two osteosarcoma cell lines using pcDNA3.1-FLAG-FBXW7 and siRNA-FBXW7. (D,E) Effect of transfection of pcDNA3.1-FLAG-FBXW7 and siRNA-FBXW7 on expression of FBXW7 and β-catenin in osteosarcoma cells MG-63 (D) and Saos-2 (E). *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant. NC, negative control; OE, overexpression.

Article Snippet: Osteosarcoma cell lines MG-63 (RRID: CVCL_0426) and Saos-2 (RRID: CVCL_0548), as well as the normal human osteoblast hFOB1.19 (RRID: CVCL_3889), were sourced from Servicebio company (Wuhan, China).

Techniques: Expressing, Transfection, Negative Control, Over Expression